P2X3 receptor expression by HEK cells conditions their survival
نویسندگان
چکیده
منابع مشابه
Embryonic expression of a P2X3 receptor encoding gene in zebrafish
From studies performed primarily in mammals, it is thought that the P2X(3) purinoreceptor is involved in mediating sensory and nociceptive signals in adult tissues. However, little is known concerning the expression or function of P2X family genes during early development. Here we describe the expression of a gene (p2x3) encoding a P2X(3) receptor during zebrafish development. We find that zebr...
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Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins. Having a higher expression level or a higher protein yield is generally desirable. As we demonstrate in this study, dropping culture temperature to 33°C, but not lower, 24 hours after transient transfection in HEK-293S cells will give rise to ~1.5-fold h...
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ژورنال
عنوان ژورنال: Purinergic Signalling
سال: 2011
ISSN: 1573-9538,1573-9546
DOI: 10.1007/s11302-011-9285-0